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SECOND EDITION New York London Taylor & Francis is an imprint of the Taylor & Francis Group, an informa microbiology, a colony- forming unit ( CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. Viable is defined as the ability to multiply via binary fission under the controlled conditions.

Counting with colony- forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all.